Tea Identification through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry
نویسندگان
چکیده
We have applied surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using titanium dioxide nanoparticles (TiO2 NPs) as the matrix and captopril (CAP) as internal standard for the determination of the concentrations of theanine and four catechins—catechin, ()-epigallocatechin (EGC), ()-epicatechin gallate (ECG), and ()-epigallocatechin gallate (EGCG). Under the optimal conditions (240 nM TiO2 NPs and 10 μM CAP), this SALDI-MS approach provides linearity of 0.3 – 80 (r = 0.990), 1.2 – 100 (r = 0.987), 4 – 120 (r = 0.995), 6 – 120 (r = 0.983), and 2 – 120 μM (r = 0.991) for theanine, catechin, EGC, ECG, and EGCG, respectively. The limits of detection (LODs; S/N = 3) for theanine, catechin, EGC, ECG, and EGCG provided by this SALDI-MS approach are 0.1, 0.35, 1.0, 1.45, and 0.5 μM, respectively. This approach provides spot-to-spot and batch-to-batch variations of less than 10% and 13%, respectively, for the analysis of tea samples. With advantages of simplicity, accuracy, precision, and great reproducibility, we have applied the SALDI-MS approach for the analysis of tea samples, with identified peaks for theanine, catechin, EGC, ECG, and EGCG. Tea samples from Taiwan and four other areas have various SALDI-MS profiles, showing their potential for differentiation of tea samples from different sources. Our result also shows that tea samples harvested in different seasons and counties in Taiwan provide significantly different MS profiles. The amounts of theanine and EGC in the Oolong tea from Lishan are much higher than those in the other tea samples.
منابع مشابه
Applications of Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight (SELDI-TOF) Mass Spectrometry in Defining Salivary Proteomic Profiles
Recent advancement in mass spectrometry leads us to a new era of proteomic analysis. Human saliva can be easily collected; however, the complexity of the salivary proteome in the past prevented the use of saliva for proteomic analysis. Here we review the development of proteomic analyses for human saliva and focus on the use of a new mass spectrometric technology known as surface-enhanced laser...
متن کاملDetection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry.
High resolution localized surface plasmon resonance (HR-LSPR) sensors were combined with matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) for the first time. LSPR sensors provide real-time label-free detection of molecular adsorption. Subsequent MALDI-MS analysis enables identification of the adsorbed molecules. This synergistic LSPR-MS approach was applied to the detect...
متن کاملTeaching Microbial Identification with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and Bioinformatics Tools†
متن کامل
Fast and reliable MALDI-TOF MS–based microorganism identification Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprinting is a fast and reliable method for the classification and identification of microorganisms, with applications
Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprinting is a fast and reliable method for the classification and identification of microorganisms, with applications in clinical diagnostics, environmental and taxonomical research, or food-processing quality control. The BioTyperTM MALDI-TOF MS fingerprinting system allows researchers to perform ...
متن کاملFast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory.
The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013